{"product":{"total":4,"offset":0,"limit":4,"data":[{"path":"/products/microplate-readers/multi-mode-readers/spectramax-m2-m2e-readers","identifier":"SpectraMax M2/M2e. Microplate Reader Shelf. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentThe SpectraMax iD3 reader offers the sensitivity and wavelength scanning capability needed to perform intrinsic tryptophan fluorescence measurements. Read mode. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The absorbance was measured at 765 nm using SpectraMax M3 Microplate Reader with SoftMax Pro 7. Abstract. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Our research provides a. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Mohd. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Then, adding 2 M sulfuric acid, the reaction was stopped, and the absorbance at 450 nm was determined using a SpectraMax M3 microplate reader (Molecular. SpectraMax DuoLuc Reporter Assay (SMP 6. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. 01) + 0. For SpectraMax L or LMax II, LMax II. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. ® reader to the computer. For more sample throughput, the system canStakMax Handling Stacker Base Unit. Click template editor icon in Home panel or select configure your plate layout in plate setup helper a. 4. Robust, high-value microplate. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataProducts : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. Ataxia telangiectasia and Rad3-related (ATR) kinase protects genome integrity during DNA replication. ” Download for Windows of SoftMax® Touch Software. 306 plate air cal fail,low light. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. spr SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Use the 2. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Bioz Stars score: 86/100, based on 1 PubMed citations. Serum IL-8 level quantification Serum IL-8 levels were quantified in sera from patients after HSCT using an enzyme-linked immunosorbent assay (Cat. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Wavelength Selection. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. In the M2, M5 and Plus. Example: [Drawer]. Luciferase Detection Instrumentation Guide. Tested w/warranty. When a feature or capability applies to only certain readers, this exception is noted. Detection modalities are shown in Table 1-1. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. , 2020). Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. Cells were transfected with Sirt1 siRNA or scrambled siRNA. The fluorescence intensity of the cells was detected using a SpectraMax M3 florescence microplate reader (Molecular Devices) with excitation and emission wavelengths of 485 and 535 nm, respectively. For more sample throughput, the system can StakMax Handling Stacker Base Unit. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and. This version of Molecular Devices SpectraMax M3 Manual compatible with such list of devices, as: SpectraMax M3, SpectraMax M4, SpectraMax M5,. 303 unable to cal dark current. 1. 90-0083 series): Excitation 530 nm,. This process should take about 3 - 5 minutes. Sale. Description 1. SpectraMax readers are the only ones on the market with the versatility to evolve and adapt to every need. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. Description 1. ATP standard curves read on SpectraMax microplate readers. 0. In the M2, M5 and Plus 384, the light. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:The bound CV was dissolved in 30% acetic acid and the absorption was measured in a microplate reader at OD 590 using SpectraMax M3 (Molecular Devices, United States). 과 같은 Parameter를 검사하여 평가할 수 있습니다. Our Price: $8,635. In addition, the total flavonoid content was determined using the aluminum chloride method. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The integration time suggested is 5-10 sec. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). To measure cell viability, 74 µl/well CyQUANT ® NF DNA binding dye was added to the cells and the cells were incubated for 60 min at 37 °C before fluorescence was measured using 485 nm excitation and 530 nm emission using a Spectramax M3 multi-mode microplate reader. The DMAC reagent was replaced by EtOH in the control wells. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] 21 Chapter 2: Set Up the StakMax Microplate Handler 4. % EPS by weight was calculated by standardization of each mean concentration of proteins, carbohydrates, and eDNA to μg/ml, then divided by total starting weight of pooled crude EPS from ten samples. U2OS cell viability (%) = [A cells with different treatments −A contro ]/[A cells with no treatments − A control ] × 100. We would like to show you a description here but the site won’t allow us. Immunofluorescence ASC antibody (1:200, Abcam, ab155970, UK) was used for immunofluorescence in P. First, 4T1 cells were. Please contact Tatiana to reserve the plate reader time: tnl1@psu. Concisely, digested test composite was treated with NaNO 2 (5%) and incubated for 5 min. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 4. Molecular Devices SpectraMax M3 Laboratory Equipment PDF Operation & User’s Manual (Updated: Wednesday 18th of October 2023 10:44:56 AM) Rating: 4. When a SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. FI assay. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax Paradigm Multi-Mode Detection Platform User Guide Loading and Unloading Microplates To load or unload a microplate: 1. Other technologies such as imaging. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. We would like to show you a description here but the site won’t allow us. Note: In this user guide, all references to SpectraMax Multi-Mode. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 1. . Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. Bioz Stars score: 96/100, based on 1 PubMed citations. The plate was placed into the SpectraMax L Luminometer and a 1-second integrated read taken of each selected well using the MycoAlert protocol in the SoftMax ® Pro Software (Read A). The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. M2. 0 out of 5 $ 12,499. 1. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. 314 low lamp level warning. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 304 signal level saturation. SpectraMax® M4. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Spectral scans were performed on native and denatured lysozyme samples using the SpectraMax iD3 reader with excitation at 270 nm and emission ranging from 300 nm to 450 nm. Find many great new & used options and get the best deals for Replacement Bulb for Molecular Devices SpectraMax M3 Flash Lamp at the best online prices at eBay! Free shipping for many products! Skip to main content. Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. DevicesThe SpectraMax M2 And M2e Systems Provide The Multi Detection Microplate Readers With Dualmonochromators, Dual-mode Cuvette Ports, With Top & Bottom Reading. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral. Learn more about its features, specifications, and applications in this user guide. Next to the pushbutton is a green LED. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Note: you might need to slide the microplate reader on the foam pad to make room for the cytometer. a SpectraMax M3 microplate reader (Molecular Devices Inc. Using. 4/5 Set up plate template / Define Plate Map 1. 3) At the bottom of the strip, click on "Show. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Data of selected analytes in the graphs below illustrate sample correlations between 96-well and 384-well format of 16 human plasma samples. LINKS to Craig…His YouTube is @BrickArmy and his business is: is COBI’s website: set in this video is #2535 M. XTplusC Texture Analyser Languages: French, English and Greek. 313 reference gain check fail. Collection in December 2013 and storage until extraction of Malaysian the A. All the experiments were done in triplicate. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. Capture Flash Assays with Ease. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Wait 20 seconds. The ALP activity level was determined, relative to that of the control group, as. Molecular Devices SpectraMax® series microplate readers. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 1. 304 signal level saturation. The SpectraMax® M4 Multi-Mode Microplate Reader dual monochromator optics allow the widest range of applications without the need to change filters and delivers single-mode performance in one multi-mode platform. Microplate Readers include the M3, M4, exception is noted. Carefully place the cytometer on its side on the foam pad so that the top of the cytometer faces the bottom of the microplate reader. Dual monochromators increase performance and assay flexibility. SpectraMax® Microplate Readers and SoftMax® Pro Software. Choose cables that meet the requirement described in this appendix. 314 low lamp level warning. 더 많은. Choose from a three- (M3), four- (M4), or SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. 7°C and the bottom to 36. Detection modalities are shown in Table 1-1. The reading was taken with a spectrophotometer (SpectraMax M3) at 640 nm. When the user applies a set temperature of 37°C, the top is heated to 37. 2. Spectral Range. The ROS level was detected by a reactive oxygen species assay kit (Beyotime, China). -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for If application. Molecular Devices Spectramax M3 Mulitmode Microplate Reader is available from The Lab World Group. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. 7°C (gradient heating design). After 20 min incubation at 37 °C in the dark, the plates were analyzed by a SpectraMax M3 microplate reader to measure the absorbance at 405 nm. Example: [Drawer]. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Microplate Readers Biotek Synergy 2 Plate. A purple product is formed when pLDH is present , and this product is quantified in a Spectramax M3 microplate reader at the absorbance of 620 nm. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Instructions for using SIRT1 siRNA and scrambled siRNA were provided by Santa Cruz Biotechnology (CA, USA). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. All procedures completely complied with the manufacturer’s instructions. Acarbose was used as a. Both microplate and cuvette ports can be qualified. Choose from a three- (M3), four- (M4), orThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Fundamentally, one is interested in whether or not an instrument can detect a signal for a given assay; that is, whether a signal is detectable above the background of the instrument. Category 2 and 3 microplate readers use gradient heating as well. 3 software (Molecular Devices, San Jose, CA, USA). Background signal was determined by adding 500 U/ml Catalase from Bovine Liver (Sigma-Aldrich, E3289) in one well to convert hydrogen peroxide into. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. , contact Molecular. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell. 312 gain calibration failed. Figure 1. Italic and boldface type are used for emphasis. As for each measurement, I. Data were expressed as mg/g gallic acid equivalents (GAE) of dry crude extract. Extramitochondrial ROS concentration was determined and compared with H 2 O 2 standards using a 96-well black plate and a spectrofluorometer SpectraMax® M3 at 540 nm emission and 580 nm. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). book Page 1 Tuesday,. The SpectraMax M2 and M2e are versatile and reliable. . The optical density was read using a SpectraMax M3 (Molecular Devices, Sunnyvale, CA, USA). 2. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. book Page 1 Wednesday, July 7, 2010 2:09 PMThe fluorescent intensity was measured using SpectraMax M3 fluorescence microplate reader (Molecular Devices) every 90 seconds with excitation at 320 nm and emission at 420 nm at 37°C for 2 hours. The SpectraMax M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for. 37) was ordered from Sigma Aldrich (St. For example, microplates containing different samp les can be read using the same orCell viability는 세포막 Integrity 또는. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. We would like to show you a description here but the site won’t allow us. Other technologies such as imaging. o For example: Ex: 485/Em 525/Cutoff 515 4. 4. The cuvette must be in place when you read the plate. Then, 25 nM HOPS complex, 50 nM Sec18, 600 nM Sec17, and finally 100 nM Vam7 were added. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 303 unable to cal dark current. Filterless readers provide consistent results with steady temperature regulation. The SpectraMax ABS Plus reader utilizes Molecular . The ProcartaPlex 384-well format was specifically developed to increase sample throughput while maintaining the robustness of a 96-well assay. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 100 readings per well gives optimal Z’ values for this assay. The guide also covers the software features, the specifications, and the accessories of the systems. Multi-Mode Microplate Readers. Summary: Accurate and reproducible A260 measurements can be made easily in SpectraMax Microplate Spectrophotometers with awareness of the optical properties of microplate materials and attention to technique. The concentration of CTC was expressed as mg catechin equivalent per g of extract. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. 1. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Choose from a three- (M3), four- (M4), orThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. Microplate Readers include the M3, M4,. When a SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Using the PathCheck® Sensor, results can be automatically normalized to a 1 cm pathlength so that the values are. Bithionol (2 μg/ml) and vancomycin (4 μg/ml) were used as controls. The optical density (OD) was read by SpectraMax M3 (Molecular Devices, Sunnyvale, CA). 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. SoftMax Pro. Description 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Learn how you can utilize microplate readers to drive landmark discoveries. Unplug the power cable from the back of the instrument. Resolution: In the GxP audit trail, the SpectraMax M3 and M4 Readers are now identified correctly. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. For each extract concentration, percentage parasite viability and. Wavelength Range. Page 26 The trademarks used herein are the. When a feature or capability applies to only certain readers, this exception is noted. 1 & 7. D 1 1. Dual monochromators increase performance and assay flexibility. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. 2. 2. With optimized reagents, option to operate the. We would like to show you a description here but the site won’t allow us. Find regional support. 0200-0729. Features. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the barriers to novel, landmark discoveries. . 1). gingivalis -treated BMDMs (MOI = 50), with or without pretreatment of OTTSP167 or Compound 50 for 2 h. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 50 0112-0115G Item Description Wavelengthrange 200-1000nm Wavelengthselection Monochromator,tunablein1nmincrementsPage 25 SpectraMax iD3 Multi-Mode Microplate Reader Installation Guide To install the transport lock on the plate drawer: 1. The relative number of viable cells was quantified by measuring the fluorescence (excitation at 560 nm and emission at 590 nm) using a SpectraMax ® M3 (Molecular Devices, San Jose, CA, USA). M5 reader microplate m3 spectramax molecular devices llc m5e biocompare quote fluorescenceMolecular devices spectramax plus 384 user manual pdf download Spectramax® m2/m2e multimode microplate readers, with softmax® proMolecular devices spectramax m5 microplate reader labx contract svc warranty available manufacturer. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). For 40 years, we have partnered with scientists to help them achieve landmark discoveries. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Category 2 and 3 microplate readers use gradient heating as well. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. item 6 White 18-SMD LED License Plate Lights For 04-06 BMW E46 LCI 325Ci 330Ci M3 Coupe White 18-SMD LED License. The information below applies to a standard 96 well plate, set to a linear shake mode. BioTek Synergy 2 SL Luminescence Microplate Reader with PC & Gen5 Software. The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is a multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. SpectraMax® Aquamax. Add to Cart. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. SpectraMax® M3. 1) EarlyTox NucView 488 Assay (6. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. Absorbance was measured using a SpectraMax M3 spectrophotometer (Molecular Devices, Sunnyvale, USA) at 340 nm, and the percentage of free amine groups consumption was calculated by the formula as follows: (1) F r e e a m i n e g r o u p s c o n s u m p t i o n % = 1 − A B × 100 where A is the absorbance of untreated FBP and B is the. Neutrophils were stimulated as described in tissue-culture multi-well plates. Offer Ends In: 5 Days 05: 02: 03. 4 kilograms). FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Then aluminum. 1step assay with SpectraMax microplate readers. Accuris SmartDrop. 0 (PLA) software (Stegmann Systems GmbH, Rodgau, Germany) to calculate relative. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Learn how to use the SpectraTest ABS2 Validation Plate, a convenient and reliable tool for verifying the performance of your absorbance microplate reader. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Figure 2-23: Bottle holder To install the bottle holder: 1. Cells were treated with potassium bromate (30 mM) to provide a pro-oxidant source required to lower the glutathione ratio, which was used as a. STAKMAX. Transfection with siRNA. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 4. For assays where cells were pre-treated with ABAH or vehicle control, cells were incubated in. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 160 多种预存实验方法可以直接调用，可以进行数据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功能，可以方便的进行各种数 据结果的展示。Popular answers (1) Sanjay Premi. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. A colorimetric assay was performed using a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA) at 450 nm. 1) Select the correct plate and the wells that you want to measure. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Multimode Reader Comparison Chart. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The intracellular ROS level was also cross-confirmed using the DCF-DA method with a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA,. SpectraMax Mini. Restart the system. Shake intensity. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. l. SpectraMax Microplate Readers. AquaMax® 4000. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. ® reader to the computer. Moffitt Cancer Center. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Fusion of liposomes was followed by content mixing of the RPLs and subsequent increase in fluorescence which was monitored in a SpectraMax M3 fluorescence plate reader (Molecular devices). SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Multi-Mode Microplate Readers. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. SpectraMax M3, M4, M5, and M5 e. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 1. The actual pathlength, d, of a solvent is found from the following equation: d cm. The plates were protected from light and incubated at room temperature for 8 min. The absorbance reading of the purple product was formed, which was an indication of the pLDH activity and, hence, the number of parasites present was quantified in a Spectramax M3 microplate reader (Abs 620). 1. 6: bioNova científica, s. After lysis of the cells, the mixture of 30 µL cell supernatant and 150 µL 30 µg/mL DTNB incubated for 25 min. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. The resulting absorbance values were plotted against time. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderWe would like to show you a description here but the site won’t allow us. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. . Then, the absorbance was measured using a spectrophotometer (Spectramax M3, Thermo Scientific, Waltham, MA, USA) at 734 nm. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Expanded dynamic range. VersaMax ELISA. 1 Defect ID: FB 2570 These two validation protocols are not included in the v5. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization Options in the instrument settings in SoftMax Pro Software. Microplate Reader Shelf. Lift up the lid over the chamber to insert or remove a cuvette. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Description 1. For more information, please visit us at per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Additionally, SoftMax® Pro Software automatically identifies the optimal excitation and emission wavelengths via the Spectral Optimization Wizard. 1. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). The spectrophotometer (SpectraMax, M3, Molecular Devices, Washington DC, USA) was used to confirm the encapsulation of gallic acid inside the PLGA nanoparticles. 9000-0756. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Add either alarmaBlue HS or alarmaBlue reagent to microplate wells (see recommended volumes) 3. Description 1. Bottom-reading is usually recommended for cell-based assay with adherent cells. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. BioTek Synergy LX Multi-Mode Microplate Reader (FLD/Lumi) Accuris MR9600 SmartReader 96 and MW9600 Smartwasher 96 Bundle, 115V. 2. Turn the instrument around so that the back of the instrument is. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Cell Degranulation. no. Also, when using a SpectraMax M4 Reader,. Spectranet customer portalPage 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. These solutions were used to measure the activity of the parasite lactate dehydrogenase (pLDH) enzyme in the cultures. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The Challenge. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 标准功. AquaMax® 2000. The luminescent signal was proportional to the amount of GSH or GSSG present. D 47 7. The sample was subsequently washed five times with 200 mL PBS.